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hypotonic buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hypotonic buffer
    Hypotonic Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hypotonic+buffer/HEPES/pm41566533-575-6-16
    Average 99 stars, based on 1 article reviews
    hypotonic buffer - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Transferring:

    Article Title: Interphase chromosome conformation is specified by distinct folding programmes inherited through mitotic chromosomes or the cytoplasm
    Article Snippet: .. The cells (10 × 10 6 ) were carefully resuspended with a broad pipette tip in 1 ml hypotonic buffer (10 mM HEPES pH 7.9, 1.5 mM MgCl 2 and 10 mM KCl) containing 0.1 mM phenylmethyl sulfonyl fluoride, 0.5 mM dithiothreitol (Thermo Fisher Scientific, BP172-25) and 1×protease inhibitor cocktail (Thermo Fisher Scientific, 78440), and incubated on ice for 25 min. ..

    Incubation:

    Article Title: Interphase chromosome conformation is specified by distinct folding programmes inherited through mitotic chromosomes or the cytoplasm
    Article Snippet: .. The cells (10 × 10 6 ) were carefully resuspended with a broad pipette tip in 1 ml hypotonic buffer (10 mM HEPES pH 7.9, 1.5 mM MgCl 2 and 10 mM KCl) containing 0.1 mM phenylmethyl sulfonyl fluoride, 0.5 mM dithiothreitol (Thermo Fisher Scientific, BP172-25) and 1×protease inhibitor cocktail (Thermo Fisher Scientific, 78440), and incubated on ice for 25 min. ..

    Article Title: Structural obstruction to full DNA replication in terminally differentiated skeletal muscle cells
    Article Snippet: .. To isolate nuclei, pellets were resuspended in Hypotonic Buffer (10 mM HEPES pH 7.5, 2 mM KCl, 2 mM MgCl 2 , 3 mM EDTA, HALT Protease Inhibitors (Thermo Scientific), 1 mM 1,4-dithiothreitol (DTT)) and incubated on ice for 30 or 60 min (MBs or MTs, respectively). ..

    Article Title: Nuclear p62/SQSTM1 facilitates ubiquitin-independent proteasomal degradation of BMAL1.
    Article Snippet: Plasmid transfection was carried out with Megatran (OriGene, TT200003) for Hela and HEK293 cells, while Lipofectamine 2000 (Thermo Fisher Scientific, 11668019) was utilized for other cells. .. After harvesting, cells (5 × 106) were washed twice with cold PBS, resuspended in 500 μL hypotonic buffer (10 mM NaCl, 3 mM MgCl 2 , 20 mM Tris-HCl, pH 7.4), and protease inhibitor mix- ture (Thermo Fisher Scientific, 87785)), and incubated on ice for 15 min. Then, 25 μL detergent (10% NP-40) was added and vortexed vigorously for 10 s. The lysates were centrifuged for 10 min at 200 × g at 4°C. ..

    Article Title: Structural obstruction to full DNA replication in terminally differentiated skeletal muscle cells
    Article Snippet: .. To isolate nuclei, pellets were resuspended in Hypotonic Buffer (10 mM HEPES pH 7.5, 2 mM KCl, 2 mM MgCl2, 3 mM EDTA, HALT Protease Inhibitors (Thermo Scientific), 1 mM 1,4-dithiothreitol (DTT)) and incubated on ice for 30 or 60 min (MBs or MTs, respectively). ..

    Article Title: Nuclear p62/SQSTM1 facilitates ubiquitin-independent proteasomal degradation of BMAL1
    Article Snippet: .. After harvesting, cells (5 × 10 6 ) were washed twice with cold PBS, resuspended in 500 μL hypotonic buffer (10 mM NaCl, 3 mM MgCl 2 , 20 mM Tris-HCl, pH 7.4), and protease inhibitor mixture (Thermo Fisher Scientific, 87785)), and incubated on ice for 15 min. Then, 25 μL detergent (10% NP-40) was added and vortexed vigorously for 10 s. The lysates were centrifuged for 10 min at 200 × g at 4°C. ..

    Protease Inhibitor:

    Article Title: Nuclear p62/SQSTM1 facilitates ubiquitin-independent proteasomal degradation of BMAL1.
    Article Snippet: Plasmid transfection was carried out with Megatran (OriGene, TT200003) for Hela and HEK293 cells, while Lipofectamine 2000 (Thermo Fisher Scientific, 11668019) was utilized for other cells. .. After harvesting, cells (5 × 106) were washed twice with cold PBS, resuspended in 500 μL hypotonic buffer (10 mM NaCl, 3 mM MgCl 2 , 20 mM Tris-HCl, pH 7.4), and protease inhibitor mix- ture (Thermo Fisher Scientific, 87785)), and incubated on ice for 15 min. Then, 25 μL detergent (10% NP-40) was added and vortexed vigorously for 10 s. The lysates were centrifuged for 10 min at 200 × g at 4°C. ..

    Article Title: A lipid plug affects K 2P 6.1(TWIK-2) function
    Article Snippet: The pellet was gently washed with Dulbecco’s phosphate buffered saline (Gibco 14190144) to remove leftover culture media, centrifuged (3500 x g for 20 min) to recover the cell pellet, and then flash frozen with liquid nitrogen for storage at – 80°C. .. The pellet from 1.8 L culture was resuspended in 50 mL hypotonic buffer (20 mM KCl, 10 mM Tris-HCl pH 8.0, phenylmethylsulfonyl fluoride (PMSF), 0.1 mg mL -1 DNase1, supplemented with Pierce protease inhibitor (Thermo Fischer Scientific) and was gently stirred on a Mono Direct Variomag magnetic stirrer (Thermo Fischer Scientific) at 4 °C for 30 min. ..

    Article Title: Nuclear p62/SQSTM1 facilitates ubiquitin-independent proteasomal degradation of BMAL1
    Article Snippet: .. After harvesting, cells (5 × 10 6 ) were washed twice with cold PBS, resuspended in 500 μL hypotonic buffer (10 mM NaCl, 3 mM MgCl 2 , 20 mM Tris-HCl, pH 7.4), and protease inhibitor mixture (Thermo Fisher Scientific, 87785)), and incubated on ice for 15 min. Then, 25 μL detergent (10% NP-40) was added and vortexed vigorously for 10 s. The lysates were centrifuged for 10 min at 200 × g at 4°C. ..

    Article Title: OCTN2 Activates a Non-Canonical Carnitine Metabolic Pathway to Promote MASH-HCC Progression and Immunotherapy Resistance.
    Article Snippet: .. Briefly, cells were resuspended in ice-cold hypotonic buffer (10 mM HEPES-KOH, pH 7.4; 10 mM KCl; Advanced Science, 2026 1.5 mM MgCl2 ; protease inhibitor cocktail, without detergents or EDTA) and gently homogenized using a Dounce homogenizer (10–15 strokes). ..



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